Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Alignment of CD89 derived amino acid sequences obtained by cloning and sequencing rhesus macaque (GenBank accession number AY386684) and cynomolgus macaque (GenBank accession number AY386690) cDNA from whole blood and comparison with the published human sequence (GenBank NM_002000). Amino acid differences are underlined. The first amino acid of the preprotein is numbered as residue 1. The mature peptide starts at residue 22. Arrows indicate distinct domains. The first two amino acids for EC1 are encoded at the end of the S2 exon. The signal peptide is encoded by both S1 and S2 sequences. Potential N-glycosylation sites, cysteines involved in disulfide bonds and arginine 209 critical for association with the FcRγ chain are bolded. Hu: Homo sapiens; Mamu: Macaca mulatta; Mafa: Macaca fascicularis.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques: Derivative Assay, Clone Assay, Sequencing

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Expression of recombinant macaque CD89 on HeLa cells. Cells were transfected with a plasmid containing full-length (a) rhesus macaque CD89 cDNA or (b) cynomolgus macaque CD89 cDNA and stained with anti-human CD89: thick line (rhesus MFI = 120·70, SD = 343·04; cynomolgus 30·35, SD = 345·04) or with isotype control mouse IgG: thin line (rhesus MFI = 8·65, SD = 7·40; cynomolgus MFI = 9·27, SD = 34·47). Untransfected HeLa cells stained with anti-human CD89: dotted line (MFI = 5·26, SD = 28·69). MFI = Mean fluorescence intensity, SD = standard deviation. Stable transfectants were used for detection of rhesus macaque CD89 and transient transfectants were used for detection of cynomolgus macaque CD89. 5000 events were counted per sample.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques: Expressing, Recombinant, Transfection, Plasmid Preparation, Staining, Fluorescence, Standard Deviation

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Alignment of the five rhesus macaque CD89 splice variants (GenBank accession number AY386684-AY386689) with full-length CD89 from the same species. Arrows indicate distinct domains. Mamu: Macaca mulatta.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Alignment of the three cynomolgus macaque CD89 splice variants (GenBank accession number AY386690-AY386693) with full-length CD89 from the same species. Arrows indicate distinct domains. Mafa: Macaca fascicularis.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Schematic representation of the complete CD89 transcript and corresponding splice variants identified in rhesus and cynomolgus macaques. Mamu: Macaca mulatta; Mafa: Macaca fascicularis.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Two-colour dot-plots of whole blood leucocytes from a representative rhesus (a–d) and cynomolgus macaque (e–h). Forward scatter (FSC) versus side scatter (SSC) (a and e); CD89 versus CD20 (b and f); CD3 (c and g); and CD16 (d and h).

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Two-colour dot-plots of whole-blood leucocytes from a representative rhesus (a–d) and cynomolgus macaque (e–h). (a and e) CD14 versus side scatter. CD14 versus CD89: (b and f) total leucocytes; (c and g) granulocytes; (d and h) monocytes. Gates used for granulocyte (R1) and monocyte (R2) populations of rhesus (a) and cynomolgus macaque (e) are shown.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques: